Fang-Gang (Kevin) Tseng(曾繁根)
Professor
Department of Engineering and System Science
Ph. D., UCLA, USA
Fields of Interest
- BioMEMS,
- Nano-biotechnology
- Nano/Micro Fluidic Systems
- MEMS IC/package
Research Statement
Prof. Fan-Gang Tseng, was graduated on 1998 from a world renowned team, UCLA Prof. Chih-Ming Ho’s group, dedicated to BioMEMS and Micro/nano fluidics system. Professor Ho was elected a member of National Academy of Engineering, USA, and a member of Academia Sinica in 1996 and 1997, respectively.Since 1999, Dr. Tseng has established a dedicated BioMEMS and Microfluidics program in National Tsing-Hua University ESS department, and pioneered several novel micro/nano devices for biomedical applications. His Ph.D. work, a high performance inkjet printhead, has been successfully transferred into a commercial product in 2004 through BenQ Inc. Prof. Tseng has published more than 40 BioMEMS related SCI Journal papers, 3 book chapters, and 90 international conference papers, as well as held 10 patents.He received several awards, including, Mr. Wu, Da-Yo Memorial Award (2005, NSC Taiwan), Four best paper/poster awards in the 7th and 9th Nanotechnology and Micro System Technology conference (2003, 2005) and the 15th as well as 28th National Conference on Applied Mechanics (1991 and 2004), respectively, one best poster award in the Conference of Micro Total Ayalysis System(mTAS 2004), NTHU young faculty research award (2002), NTHU outstanding teaching award (2002), NTHU academic booster award (2001), and NSC research award (2000).He has extensive experience on the protein chip design /fabrication/application, the design and fabrication of pico-liter fluid dosage and delivery systems, nanobiomaterial or single molecule investigation by AFM, the design and fabrication of integrated DVD pickup head and micro gyrospoce, etc.He was a candidate of Excellence Award of National Science Council in 2004.His related research efforts in the past 5 years can be categorized into the following 4 aspects:
a. Atomic Force Microscope (AFM) investigation on biomaterial [1-6]
In order to investigate/control the quality of bio-related materials for Bionano/BioMEMS applications, four AFM related techniques have been established in the past 3 years:
I. Investigation the mechanical properties of cell surface, including imaging cell surface morphology, Young’s modulus mapping in horizontal and vertical direction, and friction coefficient measurement.[1]
II. Development of calibration method for AFM spring constant [2]
III. Investigation of growth dynamic, surface hydrophobicity properties, and nanostructures, of mono/mixed Self Assembled Monolayers (SAMs) by the combination of average and statistic methods from AFM contact, taping, scratch, and lateral deflection modes to understand the basic science of SAMs growth and structures for better quality and structure control.The ultimate goal will be using the knowledge to design a functional mixed SAMs surface for controlling protein conjugation orientation on biochip surface. [3-5]
IV. AFM investigation on proteins, by imaging morphology, vertical and lateral force interaction to finding protein binding efficiency, the force between protein bond, and the distribution of functional groups in nanoscale. [5, 6]
b. Protein micro arrays [7-13]
This national project proposes a novel protein micro arrayeremploying 3-in-1 chip system, instantly delivering hundreds of biosamples in parallel for rapid sample immobilization, process, and detection. The 3-in-1 chip system includes: micro filling chip, micro stamper chip, and micro bio-reaction chip, applicable to high throughput disease diagnosis and drug screening. Reproducibility and Surface wettability for micro/nano droplet formation through micro/nano stamping process are investigated in detail and demonstrated excellent functinality.The detection of two human cancer markers (HURP and E6) has also been successfully demonstrated by the micro stamper system
c. Micro fluidics system and electrochemical sensor [14, 15]
A novel fluidic network system has been developed for immobilizing various enzymes on arrays in parallel for blood sensing.The network was fabricated by polymer-MEMS technology with plasma treatment on channel surface, and the liquids were driven by surface tension passively without external active power source.Enzyme-based electrochemical glucose sensor was employed as a model in this fluidic network, and the testing result showed precise enzyme dosage and functional sensor response.
d. Fiber optic immunoassay biosensor [16-19]
The concept of fiber optic immunoassay biosensor based on the sensing principle of Fabry-Perot interferomety and Self Assembly Monolayers (SAMs) coating technique has been demonstrated in vitro with the Rabbit-IgG and Anti-Rabbit IgG-Cy3 immunoreaction protocol. The preliminary results including the preparation of the fiber probes, the setup of the optic detection system for the interfered signal measurement, and the immunoreaction of the bio molecules on the fiber tip which can contribute to the wavelength shift of the interference spectrum. We also take the real time observation of the time response of the fiber biosensor immersing into the different kinds of anti-rabbit IgG-Cy3 target concentrations. The time response of the sensor in the current stage is around 1-2 minutes for concentration increment, not available yet for concentration decrement for which surface regeneration is desired and will be an important effort in this project.The relationship between the spectrum shift and solution concentrations is also obtained after this calibration experiment and the lowest detectable concentration of this fiber optic immunoassay biosensor is 10-12 g/ml that is more sensitive than that of the traditional ELISA detection with a limit of 10-11 g/ml.
Publications (Selected):
- Jeo-Ming Chang, Fan-Gang Tseng, and Haimei Huang, “Elasticity and Friction Measurement of Poly(dimethylsiloxane) Membrane and CCL-60 Cell with Atomic Force Microscope”, Seeing at the Nanoscale Abstracts, August 24-27, University of California, Santa Barbara, USA, p 31, 2003.
- Chun-Lung Wu, Hsin-Yi Hsieh, Fan-Gang Tseng, and Ching-Chang Chieng, “Investigation of Molecular Rearrangement by AFM Analysis of SAMs Annealing Process”, accepted by NTSI Nanotech 2005, May 8-12, Anaheim, California, 2005.
- Chun-Lung Wu, Fan-Gang Tseng, and Ching-Chang Chieng, “In-situ Study of SAMs Growth Process by Cross Analysis of AFM Height and Lateral Deflection”, accepted by NTSI Nanotech 2005, May 8-12, Anaheim, California, 2005.
- Chun-Lung Wu, Yu-Wen Lin,Yi-Lin Hsu, Fan-Gang Tseng, and 2Ching-Chang Chieng,Improvement of antibody-antigen binding efficiency by Dual Mixed Self-assembly Monolayers”, accepted by NTSI Bionano 2005, May 8-12, Anaheim, California, 2005.
- F.G. Tseng, S. W. Hung, Z.M Chang, J.W. Wu, J. K. Hwang, H.M. Huang, and C.C. Chieng, “Enhancement of E6 Protein Binding Force on Binding-Orientation-Sensitive Mixed SAMs”, accepted by NTSI Bionano 2005, May 8-12, Anaheim, California, 2005.
- S. C. Lin,F. G. Tseng, H. M. Huang, Y. C. Tsai, C. E. Ho, and C. C. Chieng, “Simultaneous Immobilization of Protein Microarrays by a Micro Stamper with Back-Filling Reservoir”, Sensors & Actuators B, 2003, in press.
- Yun-Ju Chuang, Fan-Gang Tseng, Jen-Hau Cheng and Wei-Keng Lin , “A Novel Fabrication Method of SU-8 Stacked Micro Channels By UV Dosage Control”, Sensors and Actuators A, 103, pp. 64-69, 2003.
- Fan-Gang Tseng, Kung-Hua Lin, Hui-Ting Hsu, and Ching-Chang Chieng, “A Surface-Tension-Driven Fluidic Network for Precise Enzyme Batch-Dispensing and Glucose Detection”, Sensors and Actuators B, 2003, in press.
- Chun-Jun Lin, Yu-Tai Tseng, Shih-Chang Lin, Mu-Chun Wang, Chung-Shi Yang, and Fan-Gang Tseng, “A novel in-vitro and in-situ immunoassay biosensor based on fiber-optic Fabry-Perot interferometry”, Second European Workshop on Optical Fiber Sensors, Santander, Spain, June 9-11, 2004.
- Chun-Jun Lin, Yu-Tai Tseng, Shih-Chang Lin, Mu-Chun Wang, Chung-Shi Yang, and Fan-Gang Tseng, “A novel fiber-optic Fabry-Perot immunoassay biosensor with ultra sensitivity in nano molecules detection for brain research”, submitted to Journal of Analytical Chemistry, 2004.